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. 2009 Aug 19;284(41):28319–28331. doi: 10.1074/jbc.M109.024406

FIGURE 6.

FIGURE 6.

FoxO1 and FoxO3 activate autophagy-related gene expression in cardiomyocytes. Expression of autophagy pathway genes LC3, Gabrapl1, and Atg12 was quantified by real time RT-PCR in cardiomyocytes infected with WT- or CA-FoxO1 (A) and WT- or CA-FoxO3 (B) recombinant adenovirus for 24 h. LC3, Gabarapl1, and Atg12 expression was significantly up-regulated with overexpression of FoxO1 or FoxO3 in cardiomyocytes. Atg12 is the most highly up-regulated gene in FoxO1-infected cardiomyocytes, whereas LC3 is the most highly up-regulated gene in FoxO3-infected cardiomyocytes. The base-line levels of LC3, Gabarapl1, and Atg12 relative to GAPDH expression are 0.96, 2.18, and 2.6, respectively. Significance (*) was determined by one-way ANOVA (p < 0.05; n = 3).