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Berberine treatment decreased IL-17 and IFNγ secretion in NOD mice. 11-week-old female NOD mice were orally administered with vehicle or berberine daily for 14 days. Freshly isolated splenocytes and lymph nodes cells were stimulated with 50 ng/ml phorbol 12-myristate 13-acetate and 500 ng/ml ionomycin in the presence of GolgiPlug for 5 h before intracellular staining (n = 4).
