FIGURE 6.

Sedimentation properties of PPCA, NEU1, and a complex of PPCA and NEU1. c(s) profiles of PPCA (A), NEU1 (B), the PPCA-NEU1 complex (C), and NEU1 at various concentrations (D), i.e. 1.8 (black line), 0.45 (red line), and 0.15 mg/ml (blue line) were obtained. Sedimentation-velocity profiles (fringe displacement) were fitted to a continuous sedimentation coefficient distribution model c(s). Experiments were conducted in 50 mm sodium acetate, pH 5, 100 mm NaCl at 4 °C and at a rotor speed of 50,000 rpm. The best-fit weight-average frictional ratio (f/f₀)_w was determined from the c(s) analysis, and a single value was assumed. Total loading concentrations for NEU1, PPCA, and the PPCA-NEU1 mixture were 0.58, 0.22, and 0.25 mg/ml, respectively.