TABLE 2.
PCR primers used for construction of XplA-heme mutants
| Mutant | Forward primer |
|---|---|
| A395T | TGGGGCACATGACCATCGGGTACCTGATCGCCTCG |
| Q438A | TGGACCCGCCAGCACTGTCTTTCCTGCGGTTTCCTACC |
| M322L | ATGTTCGAGGCGTTGCTGATGCAAAGCGCGGAGC |
| M318L | TGATGGAGGCGTTGTTCGAGGCGATGCTGATGCAAAGC |
| M394L | CCGTGGGGCACTTGGCCATCGGGTACCTGATCG |
| Mutant | Reverse primer |
|---|---|
| A395T | TACCCGATGGTCATGTGCCCCACGGCATAGAACACC |
| Q438A | CAGGAAAGACAGTGCTGGCGGGTCCATCCGGACCATCTCG |
| M322L | TTGCATCAGCAACGCCTCGAACATCGCCTCCATCACG |
| M318L | ATCGCCTCGAACAACGCCTCCATCACGGCGTCGGCATC |
| M394L | ACCCGATGGCCAAGTGCCCCACGGCATAGAACAC |