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. 2009 Aug 19;284(41):28467–28475. doi: 10.1074/jbc.M109.031559

TABLE 3.

Kinetic constants determined for wild-type XplA-heme and mutants determined by measurement consumption of RDX by HPLC (kcat and Km) and by spectrophotometry (KD) as detailed under “Experimental Procedures”

Enzyme KD Km kcat kcat/Km
μm μm s1 μm1·s1 ×103
Wild type 11.44 ± 0.39 10.23 ± 0.90 3.33 ± 0.07 325.0
A395T NDa 39.85 ± 6.86 0.07 ± 0.01 1.7
Q438A 67.28 ± 4.83 17.80 ± 1.80 0.35 ± 0.01 19.6
M322L 7.97 ± 0.94 12.04 ± 0.70 3.55 ± 0.05 295.0
M318L 34.32 ± 4.30 35.75 ± 13.69 0.57 ± 0.08 15.9
M394L 20.46 ± 0.47 4.94 ± 1.14 0.73 ± 0.03 148.0

a ND, not determined.