FIGURE 6.

NO inhibits detachment-induced Cav-1 down-regulation and cell death. A, H460 cells were detached and either left untreated or treated with SNP (50 μm) or DETA NONOate (50 μm) in the presence or absence of DTT (1 mm) in HEMA-coated plates. A, cell lysates were prepared and analyzed for Cav-1 protein expression by Western blotting after 12 h. Densitometry was performed to determine the relative levels of Cav-1 after reprobing the membranes with β-actin antibody. B, cells survival was determined by XTT assay after 12 h. C, morphology of cells treated with SNP (50 μm) or DETA NONOate (50 μm) for 12 h. Data are the mean ± S.D. (n = 3). *, p < 0.05 versus non-treated control; #, p < 0.05 versus the indicated treatment controls.