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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Nat Cell Biol. 2009 Nov 15;11(12):1399–1410. doi: 10.1038/ncb1986

Figure 4.

Figure 4

AMPH-1 function in endocytic recycling requires interaction with RME-1. AMPH-1(+) and interaction deficient AMPH-1(F309A, F363A) were expressed as mCherry fusions in amph-1(tm1060) mutant intestines. AMPH-1(+) ( c) but not AMPH-1 (F309A, F363A) (d) could rescue the abnormal accumulation of recycling cargo hTfR-GFP in amph-1(tm1060) mutants. Synchronized adult worms were imaged by confocal microscopy. (e) Quantification of the hTfR-GFP labeled structures in the worms with respect to average number of labeled structures per unit area. The asterisk indicates a significant difference in the one-tailed Student’s T-test, p value for WT compared against amph-1(tm1060) = 9.7×10−22 and for WT compared with amph-1(tm1060); AMPH-1(F309A, F363A)-mCherry=9.4×10−22. For all the presented data mean values were plotted on the graph and error bars represent ± s.d. from the mean. In all the experiments, n = 30 sampled fields (six animals of each genotype sampled in five different regions of each intestine). Scale bar represents 10 µm.