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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: J Mol Neurosci. 2008 Aug 3;37(2):123–134. doi: 10.1007/s12031-008-9122-2

Figure 1.

Figure 1

VPA increases bcl-2 protein levels in SH-SY5Y cells in a time- and concentration-dependent manner. SH-SY5Y cells were grown in DMEM medium supplemented with 10% FBS, antibiotics, and sodium pyridine to 80–90% confluence and treated thereafter for 8, 24, 48, 72, or 96 h with VPA (0.8 mM; VPA8, 24, 48, 72, or 96, respectively) or with vehicle control (C8, 24, 48, 72, or 96, respectively). A, C Cells were harvested and processed for Western blot analysis. B, D Densitometric results for Western blot analysis are displayed as percent of control, which was calculated as a mean of all of the controls. Data in the bar graph are means±SE from four sets of replicates. ***p<0.001; **p<0.01