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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Cancer Cell. 2002 Sep;2(3):205–216. doi: 10.1016/s1535-6108(02)00125-3

Figure 4. BM-induced tissue polarity is necessary for apoptosis resistance.

Figure 4

S−1 cells were grown either inside the rBM, inside collagen I, or inside collagen I followed by addition of either rBM or laminin-1. A: Confocal microscopy of Z sections of nuclei (propidium iodide), β-catenin (Texas red), β4 integrin (FITC), α6 integrin (FITC), and laminin-5 (FITC) fluorescence. All structures had β-catenin localized at cell-cell junctions. Although 3D structures grown in contact with collagen I had cytosolic β4 and α6 integrins and dispersed laminin-5, when these cells were overlaid with rBM or laminin-1 (not shown), their β4 and α6 integrins became reorganized to the site of cell-rBM interactions, and they assembled an endogenous BM as shown by deposition of laminin-5 at the cell-rBM junction. B: Apoptotic labeling indices calculated for S−1 cells grown as described in A. Cultures were treated with TNF-α (100 µM) for 96 hr. Results are mean ± SEM of 3–6 separate experiments, each with duplicates or triplicates. Bar equals 10 µm.