Skip to main content
. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Immunol. 2009 Jul 31;183(5):2974–2983. doi: 10.4049/jimmunol.0900495

Figure 4. NF-κB activation and c-Jun phosphorylation in TLR7 tolerant B cells.

Figure 4

Mouse high density resting B cells stimulated with R848 (1µg/ml) for 24h were washed and restimulated with R848 (1µg/ml) for indicated time points. Control B cells were incubated at 37°C for 1h in medium and then stimulated with R848 for times indicated. Cells were lysed and subjected to Western blot analysis for phospho and total IkBα (A) or p-c Jun (B). Blots were stripped and reprobed for actin as a lane-loading control.