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Affinity electrophoresis of wild-type and mutant F5/8C modules. The indicated proteins were separated by native 12% PAGE (A) and native PAGE including 0.3% (w/v) laminarin in the separation gel (B). Open arrows point to the migration positions of the wild-type F5/8C module. The relative mobility (RM) of each protein compared with BSA under the given conditions is indicated.
