Figure 8.

Dependence of the protein fraction bound to chitin on the chitin concentrations. Each of the indicated proteins (20 μg/mL) was thoroughly mixed with chitin at the indicated concentrations at 37°C for 1 h. The fractions of the protein that remaining in the supernatant (S) and co-precipitating with chitin (P) were determined by SDS-PAGE. The apparent binding affinity (Kd) was calculated using GraFit5 software. The data were averages from two independent experiments, each with triplicate samples.