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. Author manuscript; available in PMC: 2010 Jun 3.
Published in final edited form as: Oncogene. 2009 Sep 14;28(48):4306–4316. doi: 10.1038/onc.2009.280

Figure 5.

Figure 5

Contribution of Plcγ1 phosphorylation to FGFR3-mediated transformation in NIH-3T3. (a) Activation of signaling cascades in cells expressing S249C FGFR3 or S249C with a mutation in the PLCγ1 binding site (S249C+Y762F). (b) Morphology of NIH-3T3 cells expressing S249C+Y762F FGFR3 and control cells. Line bars indicate 100 μm. (c) Representative experiment showing total cell numbers reached on day 7 by NIH-3T3 cells expressing S249C and S249C+Y762F FGFR3, compared with controls (d) Fold difference in the number of colonies >200 μm formed in soft agar by NIH-3T3 cells expressing S249C and S249C+Y762F FGFR3, compared with control cells. Significant differences to control cells (transduced with an empty pFB vector) are indicated as ‘*’ (p<0.05) or ‘**’ (p<0.01)