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. 2009 Nov 30;9:13. doi: 10.1186/1471-2210-9-13

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Copyright ©2009 Lu and Carson; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Spiperone induce a rise in intracellular calcium levels. (A) Tracing of fluo-4 fluorescence in cells stimulated with 10 μM spiperone, 10 μM DTG and 2 μM ionomycin over time. Drugs were added at 30 sec. (B) Tracing of fluo-4 fluorescence in cells stimulated with increasing doses of spiperone. Drugs were added at 30 sec. (C) Pretreatment with 1 μM thapsigargin could not abolish ionomycin-induced calcium release. Drugs were added at 10 and 225 sec as indicated by the arrowheads. (D) Thapsigargin blocks the subsequent cellular calcium responses to spiperone. Intracellular Ca²⁺was recorded after stimulation with 1 μM thapsigargin and 10 μM spiperone. Drugs were added at 30 and 225 sec as indicated by the arrowheads. (E) Spiperone prevented thapsigargin-induced calcium increase. Intracellular calcium was traced after stimulation with 10 μM spiperone and 1 μM thapsigargin. Drugs were added at 30 and 225 sec as indicated by the arrowheads.