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. 2009 Nov 25;9:186. doi: 10.1186/1471-2334-9-186

Figure 1.

Figure 1

Purifying recombinant proteins by Ni2+ affinity chromatography and extracting total soluble proteins from Trypanosoma cruzi. Purified T. cruzi KMP-11 recombinant protein (KMP-11r), Trypanosoma cruzi truncated HSP-70 protein (TcHSP-70T), Trypanosoma rangeli HSP-70 recombinant protein (TrHSP-70), and crude T. cruzi lysate were electrophoresed in 12% SDS-PAGE and stained with Coomassie blue. Lane MW corresponds to molecular weight markers shown in kDa.