Table 8.
Group conservation of small and weakly polar residues at the helix-helix interface of PtMIPs and all XIPs
| Residuea | All PtMIPsb, c | Dicot XIPsc | Fungi XIPsc |
|---|---|---|---|
| T48 | T(91), S, A (95) | T(86), S (100) | T(44), S (67) |
| T55 | G(45), T(25), S, A (80) | T (5) | S(33), T, A (67) |
| A78 | A(69), S, C, T (91) | S(73), A (82) | G(100) (100) |
| G82 | G(51), A(41) (91) | A(73), S (77) | --- |
| A103 | A(76), S, T (87) | T, A (23) | T(44), A (56) |
| G107 | G(58), A(25), S, T (98) | A(50), S(32) (82) | A(78), S (100) |
| G129 | G(87), A (100) | G(100) (100) | G(100) (100) |
| A130 | S(56), A(33), G, T (100) | A(50), G(45), S (100) | G(89), A (100) |
| G133 | A(55), G(45) (100) | G(91), A (100) | A(89), G (100) |
| T172 | T(82), G, S, A (100) | T(55), S(27), A (100) | S(33), T, A, C (89) |
| S181 | A(42), S(27), G, T (84) | T, S (23) | G(100) (100) |
| G203 | G(87), A, S (100) | G(82), A (100) | G(100) (100) |
| S226 | S(60), A, C, T (93) | C(100) (100) | C(100) (100) |
| G248 | G(73), A, C, S (98) | G(95) (95) | G(44), A(33), S (100) |
| G252 | G(87), A (98) | A(77), S, G (91) | A(56), G (78) |
| A253 | A(60), G, C, S, T (98) | C(82), S, G (95) | G(33), C, T, A, S (89) |
| A256 | A(71), G (87) | --- | --- |
aResidue numbers correspond to that of spinach aquaporin SoPIP2;1 (PDB ID: 1Z98)
bGroup-based conservation is reported for all the five populus MIP subfamilies based on structure-based sequence alignment of 55 Populus MIPs
cSmall and weakly polar interfacial residues in Populus MIP sequences and their conservation (if it exceeds 25%) are given. If the conservation is less than 25%, only the residues are given. For each position, group conservation of all the five residues (Gly, Ser, Thr, Ala and Cys) is shown in bold and italics.