Table 2.
Information for nine markers that were sequenced from a set of 68 L. sativa accessions.
| Marker | EST/Contig in CGPDB | Primers (5' - 3') | Ta (°C) | Mg (mM) | Amplicon size (bp) |
|---|---|---|---|---|---|
| LK1457 | QG_CA_Contig4638 | F - AGGAGCAAAGGAAAGGCTTC | 57 | 1.5 | 636-648 |
| R - TGCAACTTCTTCAGCCAATG | |||||
| Cntg10044 | CLS_S3_Contig10044 | F - GCATGCCGATTACTCCTTTC R - TCCCCAATCACCTAAGATGG |
57 | 1.5 | 845-860 |
| QGG19E03 | QGG19E03.yg.ab1 | F - ATATCCCACCGCCCATAGAT | 57 | 1.5 | 711-720 |
| R - ACGCAACTAACCCGTTTCAT | |||||
| Cntg4252 | CLS_S3_Contig4252 | F - GGGGAGTTCAGACGTTCAGT | 57 | 1.5 | 1160 |
| R - CGAATTGATACACCGCAAAA | |||||
| Cntg10192 | CLS_S3_Contig10192 | F - CTCGTTTTCAACACCGACAA | 57 | 1.5 | 349 |
| R - TTGTCTCCGGCACTGTATCATCG | |||||
| CLSM9959 | CLSM9959.b1_N18.ab1 | F - TGCTCAATTACACTCGAACCA | 57 | 1.5 | 326 |
| R - CTTCATGGAGAGAAATACAAGGTC | |||||
| CLSZ1525 | CLSZ1525.b1_J22.ab1 | F - TTGTTGAAATTATAAACACGAAGCA | 57 | 3 | 499-629 |
| R - CAACAAAGGATGTCTCAAATTCA | |||||
| QGC11N03 | QGC11N03.yg.ab1 | F - GCACCTGATGGCTGAATATG | 57 | 1.5 | 569-581 |
| R - CATCCTCAATCGCTTGTGTT | |||||
| Cntg11275 | CLS_S3_Contig11275 | F - GGAGAAATTTTGGAGCTGTAATTAC | 61 | 1.5 | 765-956 |
| R - GGAGGTATGTTGAGGTACATGAC |
Columns indicate marker name, EST or Contig information in the CGPDB database, forward and reverse primers, annealing temperature (Ta), magnesium concentration in PCR reaction, and size of amplicon. Marker QGG19E03 could not be successfully amplified from 13 accessions even though 34 primer combinations were tested.