Table 3.
Information for six markers that were analyzed in the (Valmaine × Salinas 88) × Salinas mapping population with the HRM approach.
| Marker | EST/Contig in CGPDB | Primer (5' - 3') | Ta (°C) | Mg (mM) | Amplicon size (bp) |
|---|---|---|---|---|---|
| LK1457 | QG_CA_Contig4638 | F - AGGAGCAAAGGAAAGGCTTC | 64 | 3 | 636-648 |
| R - TGCAACTTCTTCAGCCAATG | |||||
| Cntg4252 | CLS_S3_Contig4252 | F - AGAACCAGGTCGAATCATGG | 61 | 1.5 | 208 |
| R - TTCTCGCCGTTGAGAAGAAT | |||||
| Probe - AAGTGGCTATACAGCTTTGATCATAACGA | |||||
| Cntg10192 | CLS_S3_Contig10192 | F - CTCGTTTTCAACACCGACAA | 61 | 1.5 | 185 |
| R - TAGGTGGGTCCGACTTTGAG | |||||
| CLSM9959 | CLSM9959.b1_N18.ab1 | F - TGCTCAATTACACTCGAACCA | 61 | 1.5 | 326 |
| R - CTTCATGGAGAGAAATACAAGGTC | |||||
| CLSZ1525 | CLSZ1525.b1_J22.ab1 | F - GAAGAAACTCATGAATCTGCTCAA | 62 | 3 | 157-158 |
| R - TTTGCTCAAGAACTCTTAAACCATT | |||||
| Cntg11275 | CLS_S3_Contig11275 | F - CCAAACCATAGGGACGAAAA | 61 | 1.5 | 252-260 |
| R - GGAGGTATGTTGAGGTACATGAC |
Marker Cntg4252 was analyzed in combination with a probe. Polymorphisms for three markers that are not shown in the table were detected by electrophoresis. All information for these is the same as in Table 2.