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. 2009 Nov 19;7:128. doi: 10.1186/1477-7827-7-128

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Copyright ©2009 Ricci et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pre- (2a) and post-processing (2b, 2c) cytofluorimetric analysis of a semen sample with a low leukocyte concentration. Cytogram 2a displays the cytofluorimetric analysis of a semen sample containing a low concentration of leukocytes, stained with Syto-16 and CD45 to identify sperm and leukocytes. Region R2 (blue colour) and Region 3 (red colour) represent the number of leukocytes and sperm counted, respectively. Cytograms 2b and 2c displays the cytofluorimetric analysis of the same sample after density-gradient centrifugation and after swim-up technique, respectively.