Table 2.
Hydrogen bonding pattern of SD enzyme and SHK substrate. T. thermophilus values are shown in bold, MtbSD K69A corresponding values are shown in italics.
| SD residue | SD Atom | SHK atom | H bond distance (Å) |
|---|---|---|---|
| Ser14/Ser18 | Oγ | O2 | 2.62/2.55 |
| Ser16/Ser20 | Oγ | O2 | 2.72/2.73 |
| Thr60/Thr65 | Oγ 1 | O11 | 3.28/3.22 |
| Lys64/Lys69 | Nζ | O11 | 2.80/-- |
| Lys64/Lys69 | Nζ | O12 | 3.10/-- |
| Asn85/Asn90 | Nδ 2 | O12 | 3.15/2.93 |
Atom numbering as [24]*.
* O2 corresponds to carboxyl negative oxygen atom, O11 to hydroxyl oxygen bound to C3, O12 to hydroxyl oxygen bound to C4, and O7 to hydroxyl oxygen bound to C5, as depicted in Fig. 1.