TABLE 1.

Antibodies used for staining cells to verify their identity.

Antibody	Isotype	Source	Cat. no.	Dilution
OCT4	Mouse IgG	Santa Cruz	sc-5279	1:1000
PAX6	Mouse IgG	DSHB	PAX6	1:5000
SOX1	Goat IgG	R&D	AF3366	1:1000
OLIG2	Goat IgG	Santa Cruz	SC-19969	1:500
NKX2.2	Mouse IgG	DSHB	74.5A5	1:50
NG2	Mouse IgG	BD Pharmingen	554275	1:400
PDGFRα*	Rabbit IgG	Santa Cruz	SC-338	1:400
04*	Mouse IgM	Chemicon	MAB345	1:50

PDGFRα, platelet-derived growth factor receptor alpha. After fixation with 4% (wt/vol) paraformaldehyde (PFA) for 10 min and penetrating with 0.1% (vol/vol) Triton-X, the cells are incubated with serum (donkey or goat) for 1 h to block the unspecific staining and with primary antibodies overnight at 4 °C, followed by thorough rinsing with phosphate-buffered saline (PBS) and incubation with fluorophore labeled secondary antibodies for 30 min at room temperature.

^*The cells on coverslips are incubated with primary antibodies in L15 medium for 15–20 min at room temperature before fixation, and then stained with secondary antibodies.