Table 1.
Total matched spectra |
Total non- phosphopeptide spectra (% total) |
Total phosphopeptide spectra (% total) |
Total unique phosphopeptides |
|
---|---|---|---|---|
One round TiO2 enrichment |
526 | 178 (33.8%) | 348 (66.2%) | 48 |
Two rounds TiO2 enrichment |
275 | 16 (5.8%) | 259 (94.2%) | 60 |
Albumin/IgG depletion fraction |
357 | 86 (24.1%) | 271 (75.9%) | 23 |
Albumin/IgG bound fraction |
320 | 238 (74.4%) | 82 (25.6%) | 27 |
Cell lysate | 486 | 62 (12.8%) | 424 (87.2%) | 228 |
All results were obtained using the following criteria to filter the SEQUEST search results: “ranked top #1; Xcorr versus charge 1.8, 2.5 for 2+, 3+ ions; mass accuracy 3 ppm; probability of randomized identification of peptide < 0.05”. The estimated false discovery rate by searching combined forward and reversed database is lower than 1%.