Table 2.
Serum phosphopeptides identified from two steps TiO2 enrichment and LTQ-Orbitrap analysis
| Protein description | Phosphopeptide sequence | MS2 spectra count |
|---|---|---|
| kininogen 1 | ETTCSKEpSNEELTESCETK | 124 |
| ETTCpSKESNEELTESCETK | 7 | |
| ETTCpSKEpSNEELTESCETK | 1 | |
| EpTTCSKESNEELTESCETK | 8 | |
| ETpTCSKESNEELTESCETK | 6 | |
| EpTpTCSKESNEELTESCETK | 2 | |
| serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1 |
ATEDEGpSEQKIPEATNR | 16 |
| serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10 |
VVQAPKEEEEDEQEApSEEK | 2 |
| VVQAPKEEEEDEQEApSEEKApSEEEK | 2 | |
| alpha-2-HS-glycoprotein | HTFMGVVSLGSPpSGEVSHPR | 10 |
| HTFMGVVSLGSPSGEVpSHPR | 1 | |
| HTFMGVVSLGpSPpSGEVSHPR | 1 | |
| apolipoprotein L1 isoform a precursor | VTEPISAEpSGEQVER | 4 |
| VTEPIpSAESGEQVER | 4 | |
| VTEPIpSAEpSGEQVER | 3 | |
| apolipoprotein A-II preproprotein | pSKEQLTPLIK | 2 |
| EPCVEpSLVSQYFQTVTDYGK | 1 | |
| EPCVESLVpSQYFQTVTDYGK | 1 | |
| apolipoprotein A-IV precursor | ISApSAEELR | 3 |
| apolipoprotein AV | EPApTLKDSLEQDLNNMNK | 1 |
| EPATLKDpSLEQDLNNMNK | 1 | |
| apolipoprotein A-I preproprotein | THLAPYpSDELR | 1 |
| apolipoprotein E precursor | GEVQAMLGQpSTEELR | 2 |
| inter-alpha globulin inhibitor H2 polypeptide |
SLPGEpSEEMMEEVDQVTLYSYK | 3 |
| secreted phosphoprotein 1 isoform b | ISHELDSApSSEVN | 2 |
| ISHELDpSASSEVN | 1 | |
| FRIpSHELDpSASSEVN | 1 | |
| AIPVAQDLNAPSDWDpSR | 1 | |
| secreted phosphoprotein 2, 24kDa | KDpSGEDPATCAFQR | 1 |
| NNYLFGLIpSDEpSIpSEQFYDR | 1 | |
| insulin-like growth factor binding protein 3 isoform b precursor |
YKVDYESQSTDTQNFpSSESKR | 2 |
| clusterin isoform 1 | VTTVASHTSDpSDVPSGVTEVVVK | 1 |
| VTTVASHpTpSDSDVPSGVTEVVVK | 1 | |
| fibrinogen, alpha polypeptide isoform alpha-E preproprotein |
PGSTGTWNPGpSSER | 2 |
| complement component 3 precursor | IPIEDGpSGEVVLSR | 2 |
| SGpSDEVQVGQQR | 1 | |
| pSGSDEVQVGQQR | 1 | |
| complement component 9 | AEQCCEETASpSISLHGK | 2 |
| albumin precursor | YICENQDSISpSK | 2 |
| YICENQDpSISSK | 1 | |
| heparin cofactor II precursor | GGETAQpSADPQWEQLNNK | 2 |
| coagulation factor V precursor | CIPDDDEDSpYEIFEPPESTVMATR | 1 |
| CIPDDDEDpSYEIFEPPESTVMATR | 1 | |
| YKVDYESQSpTDTQNFpSSESKR | 1 | |
| golgi phosphoprotein 2 | RQVEKEEpTNEIQVVNEEPQR | 1 |
| TFPGFFSPMLGEFVSETEpSR | 1 | |
| chordin-like 2 | PHSQNLPLDSDQEpSQEAR | 1 |
| selenoprotein P precursor | DMPApSEDLQDLQKK | 2 |
| plasminogen-related protein B2 | AFQYHpSKEQQCVIMAENR | 1 |
| hypothetical protein LOC54964 | SSAINEEDGSpSEEGVVINAGK | 2 |
| FIANpSQEPEIR | 1 | |
| vitronectin precursor | DpSWEDIFELLFWGR | 2 |
| matrix, extracellular | INQELSpSKENIVQER | 1 |
| phosphoglycoprotein with ASARM motif (bone) |
||
| angiopoietin-like 3 precursor | IDQDNSpSFDSLSPEPK | 1 |
| fibronectin 1 isoform 2 preproprotein | RPGGEPSPEGTTGQpSYNQYSQR | 1 |
| RPGGEPpSPEGTTGQSYNQYSQR | 1 | |
| chondroitin sulfate proteoglycan 2 (versican) |
QEIESETTpSEEQIQEEK | 1 |
| QEIEpSETTSEEQIQEEK | 1 | |
| SPARC-like 1 | HIQETEWQpSQEGK | 1 |
| bovine beta casein (spiked in standard) |
FQpSEEQQQTEDELQDK | 8 |