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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cancer Res. 2009 Nov 3;69(23):9038–9046. doi: 10.1158/0008-5472.CAN-09-1499

Figure 4.

Figure 4

Functional analysis of miR-129-2 in endometrial cancer cell lines. A & B, relative levels of SOX4 mRNA (A) or protein (B) expression in ECC-1 and Ishikawa cells after transient transfection with miRNAs or negative control (NC) RNA oligonucleotides for 24 or 48 h. GADPH and β-actin served as an internal control of mRNA or protein, respectively. Error bars, SD from triplicates; *, P < 0.05 compared with NC at the same time point. C, cellular proliferation by MTS assay in the endometrial cancer cell lines ECC-1 and Ishikawa transfected with miR-129-3p (3p), miR-129-5p (5p), or negative control (NC). Proliferation measured as described in Fig. 1C.