Figure 6.

A, Photon flux of xenografts over 60 days for two independent experiments in which nude mice were implanted with luminescent U87MG xenografts (U87MG-GL in a and U87MG-LucNeo in b, 5 × 10⁵ cells). In each experiment, 10 mice were left untreated and 10-20 mice underwent two treatments with “empty” hMSCs or hMSCs-Delta24 (1.5×10⁶ cells, 50MOI×24h incubation). Mice were imaged weekly. Total photonic flux was measured from fixed regions of interest encompassing the entire head. Each line represents efflux from a single animal. Slower growth is seen with hMSC-Delta24 treatment. B, Survival data corresponding to the two experiments presented in A. In Experiment 1 (a) median survival was 77 days in treated animals compared to 42 days in untreated controls and 53.5 days in animals given “empty” hMSCs. Among treated animals, 37.5% lived beyond 80 days. * P < 0.01 and ** P = 0.0002, log-rank test. In Experiment 2 (b), median survival was 60 days in treated animals compared to 45 days in untreated controls. 36% of animals lived beyond 80 days. *** P < 0.0001, log-rank test. C, Representative bioluminescence images over the time course of the experiment depicted in a. Individual mice were tracked. N, no animal. U, death unrelated to tumor. T, tumor-related death.