Figure 1.

Mouse milking procedures. a Method 1 showing milk being drawn into the Pasteur pipette from the right thoracic #3 mammary gland. b Mouse being milked using Method 2. The soft latex tubing is positioned over the teat, with milk being collected into the collection tube. c Apparatus used in Method 2. A Tygon tubing connected to house vacuum, progressively stepped down in size, B roller clamp, C rubber stopper connected to tubing via connector, and bored to receive the 1.5 ml centrifuge tube, D Multiflex tip penetrating rubber stopper, E latex teat cup, F 1.5 ml centrifuge tube. The dark blue clamp (bottom center) is an optional vacuum cut-off. d Coomassie blue-stained SDS-PAGE analysis of milks from different species and their crude fractions, as prepared by undergraduate students. The predicted proteins detected in mouse milk (as described by Boumahrou et al. [2]) are 1. whey acidic protein, 2. γ-casein, 3. β-casein, 4. αs1-casein, 5. serum albumin, 6. lactoferrin.