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Trypanothione reductase assay principle. Panel A, structure of trypanothione disulphide. Panel B, in the DTNB-coupled assay trypanothione is recycled to trypanothione disulphide by the reduction of DTNB. Formation of thionitrobenzoate anion (TNB-) is monitored at 412 nm. Panel C, in a modification of the DTNB-coupled assay NADPH is recycled by the enzyme glucose-6-phosphate dehydrogenase.
