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Analysis of trypanothione reductase purification by SDS-PAGE. Lane 1, crude cell lysate (2 μg); lane 2, protein after 35–70% ammonium sulphate cut (2 μg); lane 3, eluate from 2′5′-ADP Sepharose column (1 μg); lane 4, eluate from Q-Sepharose column (1 μg); lane 5, purified T. cruzi recombinant trypanothione reductase (0.2 μg).
