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Ki determination of TbTryR with respect to clomipramine. T(S)2 was varied as the substrate to confirm the mode of clomipramine inhibition and the Ki value. An F-test confirmed the mode of inhibition as linear competitive. Clomipramine was added at 0 μM (open circles); 1 μM (closed circles); 2 μM (open squares) and 4 μM (closed squares).
