Table 1.

Purification of recombinant T. b. brucei trypanothione reductase from E. coli. Activity was measured in the standard 340 nm assay as described in the methods.

Step	Volume (ml)	Total protein (mg)	Specific activity (U mg−1)	Total activity (U)	Purification factor x-fold	Yield (%)
Cell lysate	40	182	2.4	442	1	100
35–70% (NH4)2SO4	10.0	96.3	3.4	326	1.4	74
2′5′-ADP-Sepharose	17.8	4.5	78.4	353	32	80
Q-Sepharose	2.1	2.8	91.0	258	38	58