Fig. 7.

Cytokine release by DCs from naïve and infected MLNs. Spleens and MLN from C57BL/6 mice were taken 7 days following infection with N. brasiliensis (Nb) or H. polygyrus (Hp), and CD11c⁺ DCs isolated as described in the legend to Fig. 6. CD11c⁺ cells, pooled from 4 to 6 mice per group, were plated in triplicate at 10⁴/well with or without 1 μg/ml LPS and supernatants harvested 72 h later before analysing by ELISA. Similar results, but lower magnitude responses, were obtained from parallel cultures of splenic DCs (data not shown). Data presented are representative of two experiments with similar results. ^**p < 0.01; ^***p < 0.001.