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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Cancer Prev Res (Phila). 2009 Nov 24;2(12):1065–1075. doi: 10.1158/1940-6207.CAPR-09-0010

Fig. 1. Effect of GTP consumption on DNA methylation in wild-type (WT) mice.

Fig. 1

A) 5mdC levels in prostate, gut, and liver tissue in control or GTP-treated WT mice at 12 and 24 weeks of age was measured by LC-MS as described in Materials and Methods. B) B1 repetitive element methylation in prostate, gut, and liver tissue in control or GTP-treated WT mice at 12 and 24 weeks of age was measured by bisulfite pyrosequencing as described in Materials and Methods. C) Mage-a8 methylation in prostate, gut, and liver tissue in control or GTP-treated WT mice at 12 and 24 weeks of age by bisulfite pyrosequencing as described in Materials and Methods. Mice were administered GTPs beginning at 4 weeks of age. Organ matched DNA was used from WT or Dnmt1 hypomorphic mice in addition to in vitro methylated (M Control) and unmethylated (U Control) DNA as controls. Each symbol represents an individual sample and the bar indicates the median of each group. Mann-Whitney Test was used to determine significant differences.