Fig. 3.

CD2019 stimulates neurite outgrowth of cerebellar neurons in the presence of MAG via PI3K signalling. (A) CD2019 overcomes MAG inhibition. Cerebellar neurons were cultured over monolayers of 3T3 cells in control media (open bars) or media supplemented with the MAG-Fc at 20 μg/ml (black bars) for 21 h. In the absence of CD2019, MAG inhibits neurite outgrowth, but with increasing concentration of CD2019 this inhibition is overcome. (B) CD2019 induced loss of inhibition is counteracted by the presence of a PI3K inhibitor (LY294002 at 10 μM) but not a PKA inhibitor (KT5720 at 200 nM). (C) Western blot for phospho-Akt and total Akt and quantification. In CD2019-treated cultures there is 4-fold induction of phospho-Akt compared to control cultures but no significant increase in total Akt. Data is representative of three different cultures. Error bar shows SEM. ⁎P < 0.005, Student's t-test.