Figure 1. A portion of Multiple Alignment showing conservation and RE_M for each column.

Figure showing a representative portion of the alignment of MFS sequences and is generated using Alscript [48]. The alignment is coloured in a gradient from red to yellow on the basis of decreasing conservation score. Conservation was calculated using a method by Livingstone et al. [49] and the scores are shown as a histogram. The histogram compares the conservation, RE and the RE_M scores for the alignment columns shows in the figure. Results of selected mutations in CaMdr1p are indicated by triangles, green being sensitive while red are resistant. RE_M scores are better indicators of functional relevance than physicochemical conservation.