Figure 6. ERK1/2 deficient EC show reduced expression of Paxillin and FAK in vivo.

(A) Representative cryosections of E9.5 Erk1^−/−;Erk2^fl/fl control (left) and Erk1^−/−;Erk2^fl/fl ;Tie2-Cre (EC-DKO, right) embryos (n = 4 of each genotype) stained with anti-Paxillin. Graphic panel indicates the ratio of Paxillin positive area to total EC area in %. (B) Anti-FAK staining of representative cryosections from E9.5 Erk1^−/−;Erk2^fl/fl control (left, n = 4) and EC-DKO mutant (right, n = 4) embryo. Graphic panel indicates the ratio of FAK positive area to total EC area in %. Boxed areas are enlarged to show membrane staining in EC. The red dotted lines indicate the outer lining of the EC layer and the arrowheads point to representative EC. Lower magnification: Bars = 25 µm and Higher magnification: Bars = 20 µm.