Figure 1.

Delayed oligodendrocyte maturation in dy/dy brains. A, Mature CC1(+) oligodendrocytes per square millimeter in the corpus callosum were measured in wild-type (wt; white bars) and dy/dy (dy; black bars) littermates at 3 weeks (3W) and 6 weeks (6W) of age. Graphs depict mean (±SEM) counts obtained from 7 different areas of the corpus callosum (n = 3; *p < 0.05). B, Representative images of CC1(+) oligodendrocytes (green) in the corpus callosum of 3-week-old wild-type and dy/dy brains. Nuclei are visualized using DAPI (blue). Scale bar, 50 μm. C, Mature CC1(+) oligodendrocytes per mm² in the proximal cerebellar white matter were measured in wild-type (white bars) and dy/dy (black bars) littermates at 3 weeks and 6 weeks of age. Graphs depict mean (±SEM) counts obtained from 6 different areas of the proximal cerebellar white matter (n = 5; *p < 0.05 for comparison between 3W wt and 6W wt). D, Mature CC1(+) oligodendrocytes per mm² in the brainstem were measured in wild-type (white bars) and dy/dy (black bars) littermates at 3 weeks and 6 weeks of age. Graphs depict mean (±SEM) counts obtained from 3 different areas of the brainstem (n = 5; *p < 0.05 for comparisons between 3W wt and 6W wt, and, between 6W wt and 6W dy). E, Top panels, Representative images of MBP immunocytochemistry (green) in the corpus callosum of 3-week-old wild-type (wt) and dy/dy (dy) brains. Scale bar, 200 μm. Middle panels: representative images of MBP (green) and NF (red) immunocytochemistry in the corpus callosum of 3-week-old wild-type (wt') and dy/dy (dy') brains. Scale bar, 25 μm. Bottom panels, Representative images of MBP (green) and NF (red) immunocytochemistry in the corpus callosum of 3-week-old wild-type (wt“) and dy/dy (dy”) brains. Scale bar, 10 μm. F, Lysates from 3-week-old cerebral cortices were evaluated by Western blot to detect MBP. MBP isoforms 21.5, 18.5, 17.2 and 14.0 kDa are indicated. Actin blots are shown as a loading control.