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. 2009 Dec 16;4(12):e8334. doi: 10.1371/journal.pone.0008334

Figure 2. Effect of intracerebroventricular (icv) administration of kisspeptin antagonist (Kiss-antag) on LH pulse frequency.

Figure 2

Representative examples illustrating the effects of continuous icv infusion of (A) aCSF (4 µl/h for 3 h) or (B and C) Kiss-antag (2.5 nmol/h for 3 h) in ovariectomized 17βestradiol-replaced rats. Pulsatile LH secretion was either completely suppressed during the period of Kiss-antag infusion (B) or LH pulse interval was significantly prolonged by Kiss-antag (C). D, Summary showing the inhibitory effect of Kiss-antag on pulsatile LH secretion. P<0.001 versus aCSF control group at the same time point. #P<0.001 versus Kiss-antag treated group during the time of infusion; N = 5–6 per group. *LH pulse.