Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Dev Biol. 2009 Sep 25;336(1):53–67. doi: 10.1016/j.ydbio.2009.09.028

Figure 5.

Figure 5

Fgf10 is expressed in a gradient defined by R1 and the site of nascent Shh expression at the anterior of the RGZ. Whole mount in situ hybridization on the palatal shelves of a single embryo detecting expression of Shh (right shelf, A and B) or Fgf10 (left shelf, D and E). Dashed horizontal and vertical lines indicate approximate plane of section shown in C and F or G–N respectively (open arrowheads mark nascent ruga, black arrowheads mark definitive rugae). Section in situ hybridization for Shh and Fgf10 on adjacent sagittal (C and F) or serial frontal sections (G–N) of E13.5 wildtype palates show robust posterior expression of Fgf10 in the mesenchyme adjacent to Shh in R1 compared to the mid-RGZ (H and L), anterior RGZ (I and M), and recently formed inter-rugae domain (J and N). ps, palatal shelf; pv, palantine vein; t, tongue.