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. Author manuscript; available in PMC: 2009 Dec 7.
Published in final edited form as: J Immunol. 2008 Jan 1;180(1):637–646. doi: 10.4049/jimmunol.180.1.637

FIGURE 1.

FIGURE 1

IL-1β induces COX-2 mRNA and PGE2 in human lung fibroblasts. Subconfluent human lung fibroblasts were incubated in the indicated medium ± the indicated concentrations of IL-1β for 24 h (for PGE2 measurements) or 8 h (for RNA measurements) (n ≥ 3 in triplicate/condition). A, IL-1β induces PGE2 in a concentration-dependent manner. Conditioned medium (1.2 × 104 cells in 100 µl of medium for 24 h) was assayed for PGE2 by competitive EIA. Data are plotted as mean of nanograms of PGE2 per milliliter of conditioned medium. B, IL-1β induces COX-2 mRNA in a concentration-dependent manner. Total RNA was isolated and subjected to real-time quantitative RT-PCR for COX-2. Data are plotted as the mean ± SD COX-2 mRNA in relative to that seen in the absence of IL-1β. *, p < 0.01 by ANOVA/p < 0.05 Dunnett’s test vs SFM values.