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. Author manuscript; available in PMC: 2009 Dec 7.
Published in final edited form as: J Immunol. 2008 Jan 1;180(1):637–646. doi: 10.4049/jimmunol.180.1.637

FIGURE 2.

FIGURE 2

Induction of COX-2 mRNA and PGE2 in response to ALI edema fluid is greater than that of HYDRO fluid and is dependent on IL-1β. Subconfluent serum starved human lung fibroblasts were incubated with pulmonary edema fluid (diluted 1/20 in SFM) from patients with either ALI or HYDRO ± pretreatment with IL-1ra (200 ng/ml) or IL-1β-neutralizing Ab. A, COX-2 mRNA induction is greater in response to ALI than HYDRO, and is dependent on IL-1β. RNA (after 8 h) was quantified by real-time, quantitative RT-PCR for COX-2. Data are plotted as mean ± SD COX-2 mRNA level relative to the value observed in the presence of HYDRO fluid. *, p < 0.01 compared with HYDRO values, while + denotes p < 0.05 compared with values obtained ± IL-1ra by ANOVA. B, PGE2 secretion is greater in response to ALI than HYDRO and is dependent on IL-1β. Conditioned medium (24 h) was assayed for PGE2 by competitive EIA. Data are plotted as mean ± SD PGE2 relative to ALI + IgG control levels (3.4 ± 0.6 ng/ml). *, p < 0.001 ANOVA/p < 0.05 Dunnett’s test post hoc.