Fig.1.

Schematic diagram of minimal gus reporter gene cassette

The minimal gus reporter gene cassette contains four basic elements: a 35S promoter, the gus reporter gene, a NOS terminator, and T-DNA border repeats (LB: left border; RB: right border). The binding sites of the various primers and the unique sites, BamHI and SacI, within the minimal gus gene cassette, are shown. Primer pairs, 35S/NOS and gusF1/gusR1, were used for PCR screening; gusF1/gusR2 and gusF2/gusR2 were used for RT-PCR analysis. The 1019 bp PCR fragment amplified with gusF1 and gusR1 was used as a probe for Southern blotting analysis. The Actin gene was used as an internal reference