Fig.3.

PCR analysis of T₀ plants

Genomic DNA, isolated from the leaves, was screened for the integrity of the minimal gus reporter gene cassette by PCR using two primer pairs: 35S/NOS (2052 bp) and gusF1/gusR1 (1019 bp). M: DNA marker DL2000 (TaKaRa); Samples 1~12: transformed soybean plants (positive results are shown except in Sample 4 and Sample 9); C: the plants from the untreated flowers; P: pBI121. A similar PCR analysis was carried out in large-scale transformation with soybean plants from the flowers transformed with 0.1× SSC as the control