Fig.5.
RT-PCR analysis of gus expression in T1 plants
Total RNA of young leaves was extracted from ten independent transgenic plants. Reversed transcription (RT) was carried out with random primers according to the manufacturer’s protocol using RT-PCR kit (TaKaRa, Dalian, China). The primers gusF2/gusR2 and gusF1/gusR2 were used to generate the PCR fragments of 1019 bp and 482 bp. M: DNA marker DL2000 (TaKaRa); Samples 1~10: T1 plants; C: soybean plants from the flowers treated with 0.1× SSC; N: negative control using RT solution without total RNA as the template. An Actin gene was used as a reference molecule with the primer pair Actin-R1 and Actin-F1