Table 1.

Effects of different style cuttings on transformation frequencies and pod bearing rates in Chinese soybean cultivar genotype Liaodou 14 transformed with minimal gus reporter gene cassette

Style cutting	Flowers	Pods	PR (%)	T0 plants	PPP	TF (%) *
Uncut	200	110	55.0	310	0	0
First	213	97	45.5	179	0	0 a
Second	206	90	43.7	175	2	1.0 b
Third	209	81	38.8	135	23	11.0 d
Fourth	197	50	25.3	20	4	2.0 c

PR: pod-bearing rate; PPP: PCR positive plants; TF: transformation frequency

^* Transformants were screened by PCR using gus specific primers gusF1 and gusR1

^aindicates significance difference at the 0.05 probability level

^bindicates significance difference at the 0.05 probability level

^cindicates significance difference at the 0.05 probability level

^dindicates significance difference at the 0.05 probability level