HPLC chromatograms of hydrolysis of chlorogenic acid to caffeic acid by Viscozyme L
1: chlorogenic acid; 2: caffeic acid. Reaction: the ratio of Viscozyme L to substrate 0.02 (2 FBG), pH 3.7, 50 °C, with the reaction time of 0.5 h (a), 3.0 h (b) and 12.0 h (c)