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. Author manuscript; available in PMC: 2009 Dec 7.
Published in final edited form as: Nat Rev Mol Cell Biol. 2009 Jan;10(1):9–20. doi: 10.1038/nrm2609

Figure 3. Localization of central spindle components.

Figure 3

Simulated time course of mitotic exit of a cultured human cell line with microtubules and central spindle components labelled by indirect immunofluorescence. a| Upon anaphase onset, protein regulating cytokinesis 1 (PRC1), localizes to the overlap zone on the ends of midzone microtubules and becomes compacted during furrow ingression to form the midbody. b | The localization of mitotic kinesin-like protein 1 (MKLP1), a subunit of centralspindlin, during mitotic exit. In metaphase, centralspindlin weakly associates with the spindle. Upon initiation of chromosome segregation, MKLP1 begins to concentrate where microtubule plus ends overlap. As anaphase proceeds, MKLP1 concentrates further and its localization becomes more restricted to the very center of the central spindle. In late telophase, centralspindlin reorganizes into a ring, surrounding the midbody. c| A comparison of the distribution of MKLP1 and the chromosome passenger complex (CPC) component Aurora B demonstrates that MKLP1 localizes more discretely on the central spindle as compared to Aurora B. The cross section through the central spindle shows the prominent cortical association of the CPC, with centralspindlin and CPC labelled microtubule bundles in the centre.