Three-dimensional imaging of the Helistat® scaffolding. In 5a a Helistat® scaffolding that consists of collagen type one, is shown in an unseeded sample. In image b, the scaffolding sample has been seeded with 1,000,000 cells (slightly higher due to the looseness of the collagen) and a reduction in the porosity is noted. However, it can be seen that the largest concentrations of cells occur on the outside of the Helistat® sponge with relatively little present in the interior. In image 5c, it is noted that where 2,000,000 cells are used to seed the graft the density has increased, however, the center of the graft again still remains relatively unseeded.