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. 2009 Dec;175(6):2540–2556. doi: 10.2353/ajpath.2009.081096

Figure 6.

Figure 6

A–D: Immunoblots and respective histograms showing that IGF-II/M6P receptor levels are not significantly altered in the hippocampus (A and C) or cerebellum (B and D) of 4-, 7-, and 10-week-old (wks) Npc1−/− mouse brains compared with age-matched controls (Npc1+/+). Histograms represent quantification of the IGF-II/M6P receptor level from at least three separate experiments, each of which was replicated two to three times. E–H: Photomicrographs showing the cellular distribution of the IGF-II/M6P receptor in the hippocampus (E and G) and cerebellum (F and H) of the control (Npc1+/+; E and F) and 10-week-old (G and H) Npc1−/− mice. Note the relative change in intensity and distribution of the IGF-II/M6P receptor immunoreactivity in the hippocampus and cerebellum of Npc1−/− mouse brains. I–L: Double immunofluorescence photomicrographs of control mouse hippocampus (I and J) and cerebellum (K and L) showing the colocalization (arrows) of the IGF-II/M6P receptor (I and K) with cathepsin B (J) and cathepsin D (L) immunoreactivity. M–T: Double immunofluorescence photomicrographs of control (Npc1+/+; M, N, O, and P) and 10-week-old (Q, R, S, and T) Npc1−/− mouse hippocampus (M, N, Q, and R) and cerebellum (O, P, S, and T) showing the possible colocalization of the IGF-II/M6P receptor (M, Q, O, and S) with lectin-labeled microglia (N and R) and GFAP-labeled astrocytes (P and T). A number of astrocytes (S and T)(arrows) but not microglia (Q and R) exhibit IGF-II/M6P receptor immunoreactivity in Npc1−/− mice. Cat B, cathepsin B; Cat D, cathepsin D. IGF-II/M6PR, IGF-II/M6P receptor. Scale bar = 25 μm.