Table 1.
Primary Antibodies Used in Immunohistochemical, Immunofluorescence, and Western Blotting Experiments
| Antigen | Antibody name/clone | Host species | Dilution WB | Dilution IHC/IF | Company | Catalog no. |
|---|---|---|---|---|---|---|
| α-Synuclein | α-Synuclein | Sheep | 1/2,000 | Chemicon International, Temecula, CA | AB5334P | |
| 5-Bromo-2′-deoxyuridine | BU1/75 (ICR1) | Rat | 1/100 | Abcam | 6326 | |
| CD11B | Mac1 | Rat | 1/100 | BD Pharmingen, San Diego, CA | 550282 | |
| Cleaved caspase 3 | Asp175 | Rabbit | 1/100 | Cell Signaling Technology, Beverly, MA | 9661 | |
| ERK2 | 1B3B9 | Mouse | 1/5,000 | 1/500 | Upstate Biotechnology | 05-157 |
| Phospho-ERM | Phospho-ERM | Rabbit | 1/400 | Cell Signaling Technology | 3141 | |
| F4/80 | CI:A3-1 | Rat | 1/100 | Serotec, Oxford, U.K. | MCA497 | |
| MAP2 | AP18 | Mouse | 1/200 | Binder, L. | ||
| GFAP | GFAP | Rabbit | 1/25,000 | 1/5,000 | Dako | Z0334 |
| Nestin | nestin | Mouse | 1/50 | DSHB | Rat 401 | |
| Neurofilament dephospho | SMI 32R | Mouse | 1/1,000 | Covance Research Products, Denver, PA | SMI 32R | |
| Proliferating cell nuclear antigen | PC10 | Mouse | 1/1,000 | Oncogene | NA03T | |
| pERK | MAPK-YT | Mouse | 1/500 | Sigma-Aldrich | M8159 | |
| Tau | PHF1 | Mouse | 1/500 | ICN | PHF1 | |
| Phosphorylated neurofilament | SMI 31 | Mouse | 1/500 | Covance | SMI 31 | |
| SV2 | SV2 | Mouse | 1/10 | DSHB | SV2 | |
| Tau AT 8 | Tau AT 8 | Mouse | 1/1,000 | Pierce, Rockford, IL | MN1020 | |
| Tau AT 180 | Tau AT 180 | Mouse | 1/2,000 | Pierce | MN1040 | |
| Tau1 | Mouse | 1/1,000 | Chemicon International | 3420 | ||
| Total ERK | Rabbit | 1/5,000 | 1/500 | Sigma-Aldrich | M5670 | |
| Tubulin | Mouse | 1/5,000 | 1/5,000 | NeoMarkers | MS 581 P1 | |
| Ubiquitin | Rabbit | 1/2,000 | Dako | Z0458 | ||
| Vimentin | VIM-13.2 | Mouse | 1/2,000 | Sigma-Aldrich | V5255 | |
| CD31 | MEC13.3 | Mouse | 1/50 | BD Pharmingen | 550274 | |
| Collagen IV | Rabbit | 1/500 | Chemicon International | AB756P | ||
| F1C3 | F1C3 | Mouse | 1/1,000 | 1/200 | Faisner, A. | |
| MLC1 | MLC1 | Rabbit | 1/100 | Estevez, R. | ||
| Neurofilament | 2H3 | Mouse | Neat | DSHB | 2H3 |
WB, Western blotting; IHC, immunohistochemistry; IF, immunofluorescence.