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. 2009 Dec;175(6):2288–2298. doi: 10.2353/ajpath.2009.090474

Figure 4.

Figure 4

GRP is present in human skin (A–F) and vascular system (G–I). A–C: Sites of GRP expression determined by in situ hybridization with digoxigenin-labeled antisense probes (blue) in healthy human skin. D–F: Sites of GRP accumulation determined by immunohistochemistry with the CTerm-GRP primary antibody and peroxidase-conjugated goat anti-rabbit IgG as secondary antibody (brown), in healthy human skin. D–F show consecutive sections of A–C. Human GRP is detected in skin at the levels of epidermis (Ep) and dermis (De, A and D), small capillaries (Cp, A), and skin appendages (B, C, E, F), similarly to what is observed for rat GRP (Figures 1 and 2). Fb, fibroblast; HF, hair follicle; SwG, sweat gland. G–I: GRP accumulation in noncalcified human carotids showing GRP in vascular smooth muscle cells (VSMC) located in the tunica media (TM, G and H), and in small blood vessels (Bv) of the tunica adventitia (TA, G and I). Magnifications: A–G, ×10; H and I, ×20.